| Serum- Free Culture Media for Bovine Embryos | [Code 00000000] |
| Hihg efficiency of blastocyst formation Viable, high-quality embryos Trustful performance |
| Serum-free Culture Media for Bovine Embryo |
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IVF100 Bovine In Vitro Fertilization Medium IVF100 is an innovated bovine in vitro fertilization medium based on the BO medium (Brackett and Oliphant, 1975). IVF100 medium is developed for obtaining high efficiency of sperm capacitation and normal fertilization of bovine sperm during in vitro fertilization. The medium contains HEPES to stabilize pH change in the medium ,resulting that it can extend the long life storage of the medium. Heparin, caffeine, pyruvate, etc., containing in IVF101 medium support high efficiency of sperm capacitation and normal fertilization with matured oocytes in a short incubation (6hr). 30ml x 5pcs/set. |
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IVF110S Bovine In Vitro Fertilization Medium Set Composition of a set: - Improved type of bovine in vitro fertilization medium IVF101 10ml x 2pcs - Sperm washing solution, especially for IVF 10ml x 2pcs - Medium supplements 2pcs |
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IVMD101 Bovine Oocyte Maturation, Embryo Culture Medium (with coculture) IVMD101 is a serum-free culture medium for fully supporting bovine oocyte maturation and embryo development in vitro with a coculture of somatic cells such as granulosa/cumulus cells. This medium contains modified TCM199 supplemented with protein factors such as TGF-α, insulin, apo-transferrin, etc.,). Culture condition (5%CO2 in air) is suitable for the oocyte maturation and embryo development in vitro. 10ml x 5pcs/set |
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IVD101 Bovine Embryo Culture Medium (without coculture) IVD101 medium is a serum-free medium for fully supporting bovine embryo development in vitro without a coculture of somatic cells such as granulosa/cumulus cells. This medium contains modified TCM199 supplemented with protein factors (basic FGF, TGF-β１, aprotinin, apotransferrin, etc.,). Low oxygen culture (5%O2/5%CO2/90%N2) is highly recommended to support the high developmental potential of these embryos. 10ml x 5pcs/set |
| Serum-free culture media set for bovine embryo |
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Complete bovine embryo culture media set without coculture (for denuded
embryo) Composition : Oocyte maturation and embryo culture medium (IVMD101), Bovine in vitro fertilization medium (IVF100), Denuded embryo culture medium (IVD101), Repro C-1 plates. This set helps you to effectively produce high quality embryo without a coculture with cumulus/granulosa cell. |
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Complete bovine embryo culture media set with coculture Composition : Oocyte maturation and embryo, culture medium with a coculture (IVMD101), Bovine in vitro fertilization medium (IVF100) and Repro C-1 plates. |
| Related Media and Reagents for Bovine Oocyte Culture |
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Oocyte collection medium Optimized medium to effectively collect the healthy oocytes from the ovary. The basis of this medium is Dulbecco's Phosphate-Buffered Saline. This medium keeps an optimal pH stable when used in room temperature under an atmospheric condition. 100ml |
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Cryopreservation medium for bovine embryos BSA, Ethylene glycol, etc are included. |
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Coating solution for Repro culture plates （0.01% collagen solution） Collagen is conventionally used to sustain cell attachment and spreading of somatic cells such as cumulus/granulose cells for the embryo culture. 100ml |
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Repro C-1 plate （6-well plate, treated with collagen solution） 6-well plates of reversed conical form, optimized for in vitro fertilization and embryo culture. This plate treated with collagen is suitable for somatic cell culture with serum-free medium. Feature: 1. Suitable size for small number (less than 25) of in vitro oocyte maturation, in vitro fertilization and embryo culture 2. Effective for saving semen, culture media and mineral oil. 3. Ideal form for the observation by microscope and culture operation. 4. Collagen-coated plates promotes the cell attachment and spreading. |
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Repro plate (6-well plate without collagen coating) Same plates as Repro C-1 plates without collagen coating. Effective for the embryo / somatic cell culture and dilution of embryo freezing / thawing solution |
| Serum-free culture medium for porcine embryos in vitro |
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PZM-5 Defined Medium for Porcine Embryo Culture PZM-5 medium is a chemically defined medium suitable for porcine embryo development in vitro and in vivo and their embryos produced in this medium are able to develop to piglets after the embryo transfer (1-3). This medium supports excellent porcine embryo development without a coculture of somatic cells such as cumulus/granulosa cells under a low oxygen culture (5%O2/5%CO2/90%N2). |
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